Aishwarya Mitra

First Name: 
Aishwarya
Last Name: 
Mitra
Mentor: 
Mauricio Antunes
Abstract: 
The potential of biosensors for use in plant synthetic biology is unparalleled. In this experiment, we modified bacterial biosensors to be compatible with the plant genome in order to modulate expression of our reporter gene, Firefly Luciferase in the presence/absence of plant secondary metabolite Naringenin. In order to do so, we designed primers to insert the DNA sequence for a naringenin-binding protein into our DNA construct. By doing this and then inserting this construct into protoplasts of our model plant A. thaliana, we are able to control the expression of F Luciferase with the presence/absence of Naringenin and visualize F Luciferase's luminescence with the NightOwl Imaging System. The modulation of gene expression in plants using biosensors is a relatively unexplored concept. As such, there are few biosensors compatible with plant systems. With advances in this area of research, as this experiment aims to do, one hopes to develop optimal methods of controlling gene expression in plants via secondary metabolites, as they are accessory, or nonessential to the plant's development. The future holds promises of more efficient plant genome editing for environmental and medical applications.
Poster: 
Modulating Gene Expression Using Plant-Modified Bacterial Biosensors: An Investigation of the Regulation of Genetic Material in