Glucaric acid (GA) is a compound that was identified as a “top value added chemical” because it has diverse applications as a corrosion inhibitor, polymer strengthener, and can be catalytically hydrogenated to produce nylon. The current method for obtaining GA is by nitric acid oxidation of glucose using biocatalysts, which replaces food crops with renewable biomass crops, competing with food production. We aim to use the Methylococcus capsulatus strain as a biosynthetic pathway to convert glucose-6-phosphate (G6P) to GA. Using CRISPR/Cas9, we will incorporate the Udh, Miox4, and Ino1 genes into the pgm locus, which promotes the G6P to GA pathway.