Mentor:Dr. Jannon Fuchs
Research Topic:Characterization of P-Astrocytes in the Developing Mouse Brain
Abstract:The functions of primary cilia in multiple cell types have been intensely studied in recent years, but there is a lack of research on the primary cilia of astrocytes, which are the most abundant type of glial cell in the central nervous system (CNS). In neurogenesis, primary cilia have been shown to play an important role in cell-cycle regulation, migration and differentiation, all of which can malfunction in cancer cells. Abnormal astrocytes can give rise to a type of glioma, a brain cancer which is generally lethal and is highly resistant to known treatments. Given that, it is of great significance to study the cilia and centrosome characteristics in astrogliogenesis. Preliminary results with transgenic Aldh1L1–eGFPmice showed enhanced green fluorescent protein (eGFP) was strongly expressed in cells with astrocyte morphology. Moreover, we found that eGFPcolocalizeswith immunoreactivity for aldehyde dehydrogenase 1 family, member L1 (Aldh1L1). The next steps of this project are to examine characteristics of centrosomes and primary cilia in pre-astrocytes (p-astrocytes).
Alma Mater:B.S. Biology | University of North Texas 2015